sapi bspqi restriction enzymes (New England Biolabs)

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New England Biolabs sapi bspqi restriction enzymes
Sapi Bspqi Restriction Enzymes, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 306 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/restriction+enzymes+sapi/BspQI/pmc13035772-51-30-33
Average 97 stars, based on 306 article reviews

sapi bspqi restriction enzymes - by Bioz Stars, 2026-09

97/100 stars

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Construct:

Article Title: Efficient targeting of recombinant proteins to the thylakoid lumen in Chlamydomonas reinhardtii using a bacterial Tat signal peptide
Article Snippet: .. All other constructs were made by cutting with the restriction enzymes SapI and SphI (NEB) and subsequent ligation into pASapI/pSRSapI. ..

Ligation:

Clone Assay:

Article Title: Genetically engineered bacteria and microalgae expressing a mutant of cytochrome P450 BM3 for efficient Diuron degradation in wastewater treatment
Article Snippet: The optimized sequence was synthesized using GenScript (Piscataway, USA). .. The gene was cloned into pASapI using the restriction enzymes SapI and SphI (New England Biolabs) to create pASapIP450BM3 MT35 ( ). ..

Article Title: Genetically engineered bacteria and microalgae expressing a mutant of cytochrome P450 BM3 for efficient Diuron degradation in wastewater treatment.
Article Snippet: The optimized sequence was synthesized using GenScript (Piscataway, USA). .. The gene was cloned into pASapI using the restriction enzymes SapI and SphI (New England Biolabs) to create pASapIP450BM3 MT35 (Fig. S4). ..

Article Title: NS2 is a key determinant of compatibility in reassortant avian influenza virus with heterologous H7N9-derived NS segment
Article Snippet: .. NS1 of NS079-0702R and NS2 of NS079 fragments were then digested by the restriction enzymes SapI and XhoI (New England Biolabs, Beverly, MA, USA) and cloned into pDZ vector that was linearized by SapI, creating the plasmid designated as NS079-0702R-NS2r-pDZ. ..

other:

Article Title: Peptide library and use thereof
Article Snippet: The phagemid vector pPR3 can be used in Examples described in the paragraph (1-3) or later, instead of a phagemid vector pPR3_SPINK2(WT) constructed in the paragraph (1-2). (1-2) Construction of Phagemid Vector pPR3_SPINK2(WT) Phagemid vectors were constructed in order to display a randomly mutated SPINK2 library on phages.

Polymerase Chain Reaction:

Article Title: Production of fatty alcohols from engineered microorganisms
Article Snippet: .. The PCR product and the pCDX11-8087-rbsA-fabH plasmid were digested with the restriction enzymes SapI and BamHI (New England BioLabs, Ipswich, Mass.) following manufacturer recommendations. .. The plasmid and inserts were ligated using Quick Ligase (New England BioLabs, Ipswich, Mass.) following manufacturer recommendations and the resulting products were transformed into E. coli TOP 10 electrocompetent cells (Invitrogen, Carlsbad, Calif.) using standard techniques.

Plasmid Preparation:

Purification:

Article Title: Structural basis of DSF recognition by its receptor RpfR and its regulatory interaction with the DSF synthase RpfF
Article Snippet: .. Gibson Assembly (New England Biolabs) was used to integrate the purified insert into pTB146 [ ] and linearized using the restriction enzymes SapI and XhoI to generate the vector pHis6-SUMO-RpfR Ct (FI). pHis6-SUMO-RpfR Ct (FI) was transformed and overexpressed in E . coli strain BL21(DE3) by growing cells at 37 °C and 200 RPM in LB medium containing 100 μM ampicillin until OD 600 = 0.9. ..

Review